Transcriptome, Proteome, Histology, and Biochemistry Analysis of Oriental River Prawn Macrobrachium nipponense under Long-term Salinity Exposure

Salinity is an ecological factor affecting the physiology, survival, and distribution of crustaceans. Additionally, salinity fluctuation detrimentally affects composition biological process As a significant commercial aquaculture species in China, Japan, Southeast Asian countries, oriental river prawn, Macrobrachium nipponense, can tolerate wide range salinity. The transcriptome, proteome, histology, physiology analysis were utilized to explore physiological responses molecular mechanisms tolerance M. nipponense . Through three-month culture, statistic growth trait illustrated relatively excellent performance low salinity, higher exposure significantly affected In terms histological analysis, gills hepatopancreas suffered varying degrees damage. Besides, activities digestive, immune-related, metabolic enzymes calculated. These results indicated that influenced trypsin amylase hepatopancreas, especially 14 ppt. immune-related activated high Notably, activity was 7 ppt, which testified ppt near isotonic point gills, muscle, high-throughput mRNA sequencing revealed 11356, 2227, 1819 differentially expressed genes (DEGs) by comparing 7, 14, 21 groups with 0ppt group, respectively. TMT-labeling proteome identified 439 230 proteins (DEPs) through comparison 0 integration transcriptome several pathways related adaptation enriched, including protein export, cGMP-PKG signaling pathway, Amino sugar nucleotide metabolism, Glycine, serine threonine metabolism. 16 up down-regulated DEGs detected KEGG enrichment ETHE1, BIP, chitinase (E3.2.1.14), SARDH. no regulated recorded correlation hepatopancreas. Thus, optimum survival may from Overall, these provide valuable insights into underlying culture different